Journal: Scientific Reports

Article Title: PIC&RUN: An integrated assay for the detection and retrieval of single viable circulating tumor cells

doi: 10.1038/s41598-019-53899-4

Figure Lengend Snippet: Single cell RNA sequencing analyses. ( a ) PCA plot for single CTCs and immune cells identified using positive selection protocol. The CTC population includes cells separated using serial dilution (Control) and cells which were spiked in healthy volunteers’ blood and isolated individually using positive selection protocol (processed). ( b , c ) IPA prediction of functions based on DEGs of upregulated genes in CTCs ( b ) or immune cells ( c ). (d) PCA plot for processed Vs. Control CTCs.

Article Snippet: Principal Component Analysis (PCA) plots of RNA sequencing data of these single cells showed a clear separation between immune cells and all CTCs, regardless of the isolation procedure (RareCyte vs. serial dilution) (Fig. ).

Techniques: RNA Sequencing, Selection, Serial Dilution, Control, Isolation

Journal: Journal of molecular and cellular cardiology

Article Title: Reference glycan structure libraries of primary human cardiomyocytes and pluripotent stem cell-derived cardiomyocytes reveal cell-type and culture stage-specific glycan phenotypes

doi: 10.1016/j.yjmcc.2019.12.012

Figure Lengend Snippet: PCA analysis of N-glycan profiles from heart tissue homogenate (n=3), primary enriched CM (donor matched, n=3), and hiPSC-CM (n=3 for each time-point). A PCA plot of total N-glycome profiles for all sample types covering 93% of all observed variation. B Loading plot showing major N-glycan structure contributors to each cluster plotted with vectors (line length and angle) proportional to PCA dimension contribution strength and direction.

Article Snippet: The PCA plot of three samples from enriched CM, heart tissue homogenate and timepoints D20, D60, D100 of hiPSC-CM was generated in KNIME [ 36 ] using the PCA node using all quantified glycan structures for each sample.

Techniques: Glycoproteomics